LMX Broth, Modified
 
Catalog Number: AS-1277
 
LMX Broth, modified, was created to quickly identify and distinguish between E. coli and total coliforms. Manafi and Ossmer improved upon the original formulation of Kneifel and Manafi. Phosphate buffers promote the growth of coliform bacteria, whereas lauryl sulfate inhibits Gram-positive bacteria. The simultaneous detection of E. coli and total coliforms is made easier by the addition of chromogenic and fluorogenic substrates. Coliforms are indicated by a color shift from yellow to blue-green, and E. coli is confirmed by blue fluorescence when exposed to UV light. Enzyme activity is increased by tryptophan and 1-isopropyl-β-D-1-thio-galactopyranoside, which also raises the sensitivity and specificity of the medium.
 
Storage
Store dehydrated medium and prepared medium at 2-8 °C.