HEPES Buffer – High-Purity Molecular Biology Grade

Catalog Number: ASC-1058
Molecular Formula: C8H18N2O4S
Molecular Weight: 238.31 g/mol
CAS Number: 7365-45-9

Unlock precision in your lab with Ausamics Pty Ltd’s HEPES Buffer (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), a premium buffering agent designed for molecular biology and cell culture applications. Known for its exceptional pH stability, HEPES is the perfect choice for maintaining optimal conditions in your experiments. Whether you’re culturing cells, conducting biochemical assays, or teaching cutting-edge science, our lab-grade HEPES ensures reliable results every time. Order now and power your research with Ausamics!

Key Features

pH Stability: Maintains a stable pH range of 6.8–8.2, ideal for sensitive biological systems.
High Purity: Lab-tested to ensure low impurities, delivering consistent performance.
Solubility: Highly soluble in water, making it easy to prepare buffer solutions.

Why Choose Ausamics HEPES Buffer?

Superior Quality: Rigorously tested to meet stringent standards, ensuring reliable buffering for cell culture and molecular biology.
Trusted by Professionals: Backed by 100% positive feedback from researchers, educators, and lab managers worldwide.
Versatile Applications: Perfect for a wide range of experiments, from cell culture to protein analysis.
Fast Global Shipping: Reliable delivery via trusted couriers, with Australia Post for Australian orders.
Certificate of Analysis: Each batch includes a CoA for quality assurance, compliant with ISO 17025 standards.

Applications

HEPES Buffer is a zwitterionic buffering agent widely used in research and education:

Cell Culture: Maintains stable pH for mammalian cell lines, ensuring healthy growth and viability.
Molecular Biology: Supports biochemical assays, enzyme studies, and protein purification by stabilizing pH.
Microbiology & Mycology: Complements products like Bacteriological Peptone and Agarose Powder for environmental and food testing.
Educational Labs: Ideal for university labs and science workshops, simplifying complex experiments for students.

How It Works

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a synthetic buffer that resists pH changes caused by CO2 or temperature fluctuations, making it superior to traditional buffers like Tris or phosphate. Its zwitterionic nature ensures minimal interference with biological processes, providing a stable environment for cells, enzymes, and nucleic acids. Simply dissolve in water, adjust to your desired pH, and use in your experimental setup.

Instructions for Use

Dissolve HEPES powder in deionized water to prepare a stock solution (e.g., 1M).
Adjust pH to the desired range (typically 7.2–7.8 for cell culture) using NaOH or HCl.
Sterilize by filtration for cell culture applications.

Safety: For lab use only. Handle with gloves and follow the provided Safety Data Sheet.

Storage: Store in a cool, dry place, away from moisture and direct sunlight.