Casein-peptone Lecithin Polysorbate Broth Base - TAT Broth
Catalog Number: AS-1357
 
Used for cultivating microorganisms from highly viscous or gelatinous materials (with added polysorbate 20).
Casein peptone lecithin polysorbate broth base, also known as TAT Broth, is a neutralizing broth that is mostly used for sample dilution in cosmetics, pharmaceuticals, and other related fields. The medium is developed to facilitate the recovery of injured microorganisms by providing the best conditions possible for their development and recovery. A rich amount of nutrients is provided by the addition of casein peptone, which aids in the healing of injured cells. To neutralize different antimicrobial substances like phenols and quaternary ammonium compounds, lecithin and polysorbate 20 work as neutralizing agents. Since thioglycolate can obstruct the inactivation of some preservatives, it is purposefully left out.
 
Applications
1. Pharmaceutical and Cosmetic Industries
Microbial Limit Testing: Assesses the microbial content of non-sterile products to ensure safety and compliance.
Preservative Efficacy Testing: Evaluates the effectiveness of antimicrobial preservatives in formulations.
Environmental Monitoring: Recovers microorganisms from surfaces, equipment, and cleanroom environments.
2. Food and Beverage Industry
Surface Sampling: Detects and enumerates microorganisms on food contact surfaces and equipment.
Neutralization of Disinfectants: Overcomes residual sanitizers that may inhibit microbial growth, ensuring accurate counts.
3. Water and Wastewater Analysis
Microbial Recovery: Facilitates the detection of bacteria in water samples for quality control and regulatory compliance.
4. Research and Development
Antimicrobial Testing: Serves as a medium for studying the efficacy of disinfectants and sanitizers.
Microbial Enumeration: Supports general microbial growth for various laboratory applications.
 
Storage: Keep the container at 15-30 °C and prepared medium at 2-8 °C.