Tryptic Soy Agar (TSA, CASO Agar): The Universal Culture Medium for Microbiological Excellence
CAT No.: AS-1370
Introduction
Tryptic Soy Agar (TSA), also known as CASO Agar, is a highly nutritious, general-purpose medium widely utilized in microbiology for the cultivation and isolation of a broad spectrum of microorganisms. Renowned for its versatility, TSA serves as a foundational medium in clinical, pharmaceutical, environmental, and industrial microbiology labs globally. Its balanced formulation supports the growth of bacteria, yeasts, and molds, making it an indispensable tool for microbial analysis and quality control.
Key Features
Universal Application: Supports the growth of a wide variety of microorganisms, including fastidious species.
Nutrient-Rich Composition: Contains digest of soybean meal and casein, providing essential amino acids, peptides, and vitamins.
Versatile Usage: Ideal for microbial enumeration, isolation, and routine cultivation.
High Clarity and Consistency: Produces clear media facilitating easy observation and colony differentiation.
Quality Assurance Compliance: Manufactured to meet international standards like ISO, ensuring reliability and reproducibility.
Composition
| Ingredient | Concentration (g/L) |
|---|---|
| Tryptone (Casein Digest) | 15.0 |
| Soytone (Soybean Digest) | 5.0 |
| Sodium Chloride | 5.0 |
| Agar | 15.0 |
| Total | 40.0 |
Applications
1. Clinical Microbiology
Pathogen Isolation: Used for cultivating clinically significant bacteria from patient specimens.
Antibiotic Sensitivity Testing: Serves as a base medium for assessing microbial response to antibiotics.
2. Pharmaceutical and Cosmetic Industry
Microbial Limit Testing: Ensures products meet microbial quality specifications.
Environmental Monitoring: Assesses cleanliness in production areas through surface and air sampling.
3. Food and Beverage Industry
Quality Control: Monitors microbial contamination in food products and production environments.
Total Viable Count: Determines the total bacterial load in samples for safety assessment.
4. Environmental Microbiology
Water Testing: Evaluates microbial contamination in drinking water and wastewater.
Soil and Air Analysis: Cultivates microorganisms present in environmental samples for ecological studies.
5. Research and Education
Microbial Physiology Studies: Provides a controlled environment for studying microbial growth characteristics.
Teaching Laboratories: Widely used in academic settings for training in microbiological techniques.
Benefits
Enhanced Growth of Diverse Microbes: Supports both aerobic and anaerobic organisms, including obligate anaerobes with proper modifications.
Consistency and Reliability: Provides reproducible results critical for quality control and compliance.
Easy Preparation: Simplifies laboratory workflows with straightforward preparation procedures.
Customizable: Can be supplemented with blood or other additives to create enriched media for fastidious organisms.
Preparation Instructions:
Weigh the Medium: Dissolve 40 grams of TSA powder in 1 liter of distilled or deionized water.
Heat to Dissolve: Gently heat with frequent agitation until the medium is completely dissolved.
Sterilization: Autoclave at 121°C for 15 minutes at 15 psi pressure.
Cooling: Cool the sterilized medium to 45-50°C.
Pouring Plates: Aseptically pour into sterile Petri dishes.
Solidification: Allow the agar to solidify at room temperature.
Storage: Store prepared plates inverted at 2-8°C if not used immediately.
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