Patent Blue V, Patent Blue V calcium salt (C.I. 42051)– High-Purity Biological Stain and Colorant

CAT No.: ASD-628 | CAS: 3536-49-0 | CI: 42051 | EC No.: 222-573-8

Patent Blue V (Acid Blue 3) is a synthetic triphenylmethane dye used in biological staining, food coloring, and diagnostic applications.

Scientific Details

Chemical Name: Patent Blue V calcium salt
IUPAC Name: Ethanaminium, N-[4-[4-(diethylamino)phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-, inner salt, calcium salt (2:1)
Molecular Formula: C₂₇H₃₄CaN₂O₇S₂
Molecular Weight: 602.77 g/mol
Appearance: Dark blue to green crystalline powder
Solubility: Highly soluble in water (up to 20.98 g/L at 20 °C)
Absorption Maximum: ~640 nm
Form: Solid, hygroscopic
Storage: +20 °C, protected from light and moisture

Applications

Biological Staining: Used in histology and cytology for tissue visualization
Microbiology: Applied in chromogenic media and bacterial identification
Medical Diagnostics: Approved for sentinel lymph node mapping in surgical procedures
Food Industry: Known as E131 (Food Blue 5) in Europe; used in gelatin desserts, sweets, and beverages
Veterinary & Feed Additives: Authorized for non-food-producing animals under EFSA guidelines

Regulatory & Safety

Regulatory Status:
- Listed as E131 under EU food additive regulations
- Complies with JECFA, EFSA, and TGA standards for approved uses
Handling Precautions:
- For laboratory, diagnostic, or permitted food use only
- Avoid inhalation and contact with eyes
- Use PPE and refer to SDS for safe handling
Packaging: 10 g, 50 g, 100 g