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Neutral Red

 

Catalog Number: ASC-1035

CAS Number: 553-24-2

MW: 288.78 g/mol

MF: C15H17ClN4

 

Neutral red is a versatile dye with applications in both histology and microbiology. As a pH indicator, it changes color from red to yellow within a specific pH range (typically 6.8-8.0). This property makes it valuable in microbiology for differentiating bacteria based on their ability to ferment. In MacConkey agar, for example, neutral red along with lactose helps identify lactose fermenters. When bacteria ferment lactose, they produce lactic acid, lowering the surrounding pH. This acidic environment causes the neutral red to remain red in lactose-fermenting colonies, contrasting with the pale colonies of non-fermenters where the neutral red appears yellow due to the higher pH.

Neutral Red

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  • Cat. Number ASC-1035
    CAS Number 553-24-2
    MDL Number MFCD00012651
    PubChem 310277720
    Molecular Weight 288.78 g/mol
    Molecular Formula C15H17ClN4
    Storage Temperature +20°C
    Form and Color Powder, Green, Brown or Black
    Solubility (0.1% in 50% EtOH + 2ml HOAc) Clear, Red Solution
    Wavelength of Maximum Absorption (λmax) 535.0 – 545.0 nm
    Absorptivity (1%/1cm) ≥ 1350 (at λmax)
    Loss on Drying ≤ 10.0% (1h, 110°C)
    Dye Content (Spectrophotometry) ≥ 90.0%

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