Brilliant Green Agar (BGA) – ISO 6579-1 Reference Medium for Salmonella Isolation
CAT No.: AS-1150
 
Overview
Brilliant Green Agar (BGA) is a highly selective solid culture medium designed for the isolation and differentiation of Salmonella spp. (excluding S. Typhi and S. Paratyphi) from food, feed, and environmental samples.
It is the classical reference plating medium used globally and remains the mandatory second selective agar in ISO 6579-1:2017, used in parallel with XLD or equivalent media. BGA is widely adopted across Europe, Asia, and international food safety laboratories, particularly in poultry, egg, and meat testing.
Ausamics Brilliant Green Agar is manufactured to meet the performance expectations of ISO, DIN, and international reference methods, comparable to leading brands such as Difco™, Merck™, and Oxoid™.
 
Applications
Direct plating after selective enrichment
(Rappaport-Vassiliadis, Selenite Cystine, MKTTn)
Official second selective agar in:
ISO 6579-1:2017 (Annex D)
DIN 10160
Multiple national and regional Salmonella testing methods
Routine Salmonella screening in:
Poultry & egg products
Meat & meat products
Food processing environments
Environmental monitoring samples
 
Key Features & Selectivity
Very strong inhibition of competing flora
(E. coli, Proteus, Pseudomonas) via:
Brilliant Green dye
Phenol Red indicator system
Clear and characteristic Salmonella colony morphology
Reliable differentiation based on lactose/sucrose fermentation
Performance aligned with Edel–Kampelmacher formulation
 
Colony Characteristics
(18–24 hours at 35–37 °C)
Organism
Appearance
Salmonella spp. (non-typhi)
Pink-red to red colonies, 1.5–4.0 mm, smooth; H₂S-positive strains may show greenish-black centres
E. coli / Coliforms
Strongly inhibited or very small yellowish colonies
Proteus / Pseudomonas
Inhibited or tiny pale colonies
 
Composition (per litre)
(Modified Edel–Kampelmacher formulation)
Component
Quantity
Yeast Extract
3.0 g
Peptone / Proteose Peptone
10.0 g
Lactose
10.0 g
Sucrose
10.0 g
Sodium Chloride
5.0 g
Phenol Red
0.08 g
Brilliant Green
0.0125 g (12.5 mg)
Agar
12.0–15.0 g
Final pH: 6.9 ± 0.2 at 25 °C
 
Preparation Instructions
Suspend 50.0 g of dehydrated medium in 1 L purified water
Heat with frequent agitation and boil for 1 minute
DO NOT AUTOCLAVE
(Brilliant Green is heat-labile and excessive heat reduces selectivity)
Cool to 45–50 °C, mix thoroughly
Pour plates and allow to solidify
 
Storage & Stability
Dehydrated medium:
Store in a cool, dry place below 30 °C, protected from light
Prepared plates:
Store at 2–8 °C, protected from light
Use within 7 days
 
Quality & Compliance
Conforms to ISO 6579-1:2017 performance requirements
Manufactured under controlled conditions with batch traceability
Suitable for routine, regulatory, and accredited food microbiology laboratories
 
HS Tariff Code
HS Code: 3822.00
(Prepared diagnostic or laboratory reagents)
 
Comparison with Leading Brands
Feature
Ausamics BGA
Difco™
Oxoid™
HiMedia™
ISO 6579-1 compliant
✅
✅
✅
✅
Strong inhibition of coliforms
✅
✅
✅
✅
Edel-Kampelmacher formulation
✅
✅
✅
✅
Clear Salmonella morphology
✅
✅
✅
✅
Cost-effective supply
⭐⭐⭐⭐
⭐⭐
⭐⭐
⭐⭐⭐
Local Australian availability
✅
❌
❌
❌
 
Why Buy from Ausamics?
Australian-based supplier with fast local dispatch
Transparent documentation & technical support
Competitive pricing without compromising reference performance
Trusted by food, environmental, and microbiology laboratories
 
Intended Use & Disclaimer
For food and environmental microbiology use only.
Not intended for diagnostic or clinical use in humans or animals.
 
Official References
ISO 6579-1:2017, Annex D
Edel, W. & Kampelmacher, E.H. (1969)