Bifidobacterium Broth — CAT: AS‑1139 | Nutrient Medium for Bifidobacterium infantis
 
Overview
Bifidobacterium Broth (CAT: AS‑1139) is a nutrient‑rich liquid culture medium formulated for the cultivation and maintenance of bifidobacteria, particularly Bifidobacterium infantis. Its balanced composition of peptones, yeast extract, tomato solids, glucose, and Tween 80 provides essential amino acids, vitamins, carbohydrates, and growth factors. Optimized at pH 6.8 ± 0.2, this broth supports robust bifidobacterial growth under anaerobic conditions, making it ideal for clinical microbiology, probiotic quality control, and gut microbiota research.
 
Applications
Cultivation of Bifidobacterium infantis and related species.
Maintenance of bifidobacteria cultures in clinical and veterinary microbiology.
Quality control of probiotic formulations and fermented dairy products.
Research in gut microbiota, functional foods, and probiotics.
 
Key Features
Rich nutrient profile: tryptone, peptone, yeast extract, tomato solids.
Energy sources: glucose + tomato juice for enhanced bifidobacteria recovery.
Tween 80: improves membrane permeability and growth efficiency.
Optimized pH (6.8 ± 0.2) for bifidobacteria cultivation.
Standardized formulation (M1395) for reproducibility across laboratories.
 
Composition (per liter)
Ingredient
g/L
Function
Tryptone
20.0
Nitrogen source, peptides
Peptone
10.0
Amino acids, growth factors
Yeast Extract
10.0
Vitamins, nucleotides
Tomato Juice Solids
16.65
Organic acids, nutrients
Dextrose (Glucose)
20.0
Energy source
Polysorbate 80 (Tween 80)
2.0
Growth enhancer, surfactant
Agar (optional solid base)
15.0
Solidifying agent (if required)
Final pH
6.8 ± 0.2
Optimized for bifidobacteria
 
Preparation
Suspend 78.65 g of dehydrated medium in 1 L purified/distilled water.
Heat if necessary to dissolve completely.
Dispense into tubes or flasks.
Sterilize by autoclaving at 121 °C (15 lbs pressure) for 15 minutes.
 
Storage & Stability
Dehydrated medium: Store below 30 °C in a tightly closed container.
Prepared broth: Store at 2–8 °C; use before expiry date.
 
Safety & Compliance
For Research Use Only — Not for Human or Animal Consumption.
SDS, COA, and TDS available for download.
Conforms to ISO 29981 recommendations for bifidobacteria cultivation.
 
FAQ
Q1: What organisms is this broth optimized for? A1: Bifidobacterium infantis and related bifidobacteria species.
Q2: Can this broth be used for probiotics QC? A2: Yes, it is widely used in probiotic and dairy product quality control.
Q3: Is this formulation ISO‑compliant? A3: Yes, it aligns with ISO 29981 standards for bifidobacteria cultivation.