Amikacin Sulfate Powder: High-Purity Antibiotic for Microbiological Applications
 
Catalog Number: AS-2045 
 
Introduction
Discover the exceptional potency of Amikacin Sulfate Powder, a powerful, broad-spectrum aminoglycoside antibiotic derived from kanamycin A. Recognized for its effectiveness against a wide range of Gram-negative and some Gram-positive bacteria, Amikacin Sulfate is indispensable in microbiological research, pharmaceutical formulations, and cell culture applications. Our high-purity Amikacin Sulfate Powder ensures consistent performance and reliability for your critical projects.
Key Features
Broad-Spectrum Antibacterial Activity: Effective against numerous Gram-negative bacteria, including strains resistant to other aminoglycosides.
High Purity and Quality: Manufactured under stringent quality controls to guarantee purity and potency.
Soluble and Stable: Easily soluble in water, forming stable solutions suitable for various applications.
Versatile Use: Ideal for microbiological assays, cell culture selection, and pharmaceutical formulations.
Compliance: Meets USP (United States Pharmacopeia) standards, ensuring quality and consistency.
Chemical Information
Chemical Name: Amikacin Sulfate
Chemical Formula: C22H43N5O13.2H2SO4
Molecular Weight: 781.75 g/mol
CAS Number: 39831-55-5
Appearance: White to off-white crystalline powder
Solubility: Freely soluble in water; practically insoluble in alcohol and acetone
Applications
1. Microbiological Research
Selective Agent: Used in culture media to select for amikacin-resistant microorganisms or to suppress contaminant growth.
Antimicrobial Testing: Serves as a standard in susceptibility assays to evaluate the efficacy of new antibiotics.
Mechanism Studies: Facilitates research on bacterial protein synthesis inhibition and aminoglycoside resistance mechanisms.
2. Cell Culture
Contamination Prevention: Added to cell culture media to prevent bacterial contamination, ensuring the integrity of cell lines.
Gene Selection Marker: Utilized in genetic engineering as a selection agent for cells expressing aminoglycoside acetyltransferase enzymes.
3. Pharmaceutical Manufacturing
Active Pharmaceutical Ingredient (API): Incorporated into injectable formulations for the treatment of severe bacterial infections.
Topical Preparations: Used in creams and ointments for skin infections caused by susceptible bacteria.
4. Veterinary Medicine
Animal Health: Employed in formulations to treat bacterial infections in livestock and companion animals.
Advantages
High Efficacy: Effective against antibiotic-resistant bacterial strains, expanding treatment options.
Reliable Quality: Consistent activity ensures reproducible results in research and manufacturing.
Easy Handling: Soluble powder form allows for straightforward preparation of stock solutions.
Regulatory Compliance: Produced in facilities adhering to GMP (Good Manufacturing Practices), meeting global regulatory requirements.
Usage Guidelines
Preparation of Stock Solutions
Dissolution:
Dissolve Amikacin Sulfate Powder in sterile distilled water.
Typical concentration for stock solutions is 50 mg/mL, but adjust according to application needs.
Sterilization:
Filter-sterilize the solution using a 0.22 μm membrane filter to ensure sterility.
Storage:
Aliquot and store stock solutions at -20°C.
Avoid repeated freeze-thaw cycles to maintain antibiotic activity.
Recommended Working Concentrations
Cell Culture:
Commonly used at concentrations ranging from 50 to 100 μg/mL in culture media.
Microbiological Media:
Concentrations may vary based on the specific application and microbial susceptibility.
For Laboratory Use Only.