King’s B Medium Base (KB Medium) | Fluorescein Production Medium for Pseudomonas

King’s B Medium Base (Pseudomonas Agar F / Flo Agar)

Fluorescein-Enhancing Medium for Pseudomonas Species

Catalog Number: AS-1260

Overview

King’s B Medium Base is a selective and differential culture medium used for the cultivation and differentiation of fluorescent Pseudomonas species, particularly Pseudomonas aeruginosa, through the enhancement of fluorescein (pyoverdin) pigment production.

Also known as Pseudomonas Agar F or Flo Agar, this medium is widely used in water, environmental, agricultural, and research laboratories. It supports pigment production that can be observed as a yellow-green fluorescence under UV light, enabling visual differentiation of fluorescent Pseudomonas species.

King’s B Medium is referenced for use in water testing workflows as described in ISO 16266, particularly for samples with low background flora, such as bottled and treated waters.

Ausamics King’s B Medium Base is manufactured for consistent performance, reliable pigment expression, and reproducible results.

Key Features & Benefits

• Enhances production of fluorescein (pyoverdin) pigment

• Supports differentiation of fluorescent Pseudomonas species

• Fluorescence visible under UV light

• Suitable for water, environmental, and agricultural samples

• Widely referenced in international water testing methods

• Reliable batch-to-batch consistency

Typical Applications

Water & Environmental Microbiology

• Cultivation of Pseudomonas species from water samples

• Screening of bottled, treated, and environmental waters

• Use in workflows aligned with ISO 16266 guidance

Agricultural & Plant Research

• Detection and subculturing of fluorescent pseudomonads from plants and seeds

• Study of plant-associated Pseudomonas species

Research & Teaching Laboratories

• Pigment production and metabolic studies

• Differentiation of Pseudomonas strains based on fluorescence

• Demonstration of selective and differential media

Principle of the Medium

King’s B Medium Base contains nutrients that promote bacterial growth while enhancing the production of fluorescein, a yellow-green pigment produced by certain Pseudomonas species. When incubated and examined under ultraviolet light, fluorescent strains exhibit a bright green fluorescence, allowing differentiation from non-fluorescent organisms.

Storage & Stability

Store dehydrated medium at 10–25 °C, protected from light and moisture, in a tightly sealed container.

Do not use beyond the expiry date stated on the label. Discard the product if there is any evidence of contamination or deterioration.

Intended Use

For laboratory research and quality-control use only.

Not for human, animal, diagnostic, or therapeutic use.

Quality & Documentation

• Manufactured under controlled conditions for batch consistency

• Certificate of Analysis (COA) available

• Safety Data Sheet (SDS) available upon request