Eosin Methylene Blue Agar, EMB
 
Catalog Number: AS-1223
 
Enterobacteriaceae family members are isolated and differentiated using Eosin Methylene Blue (EMB) agar, a selective and differential culture medium. Lactose and sucrose are added to the medium as carbohydrate substrates, along with methylene blue and eosin Y dyes. These elements allow lactose and/or sucrose fermenters to be distinguished from non-fermenters. Dipotassium phosphate stabilizes pH while peptones supply vital nutrients. Because of dye precipitation, coliform bacteria display distinctive blue-black colonies; Escherichia coli frequently produces a metallic sheen. Colonies of non-fermenting organisms are usually colorless.
 
Storage
Keep the container at 15-30 °C and prepared medium at 2-8 °C.