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Czapek‑Dox Broth, AS-1189
$27.00 AUD
AuSaMicS Life Science™ · Dehydrated Culture Media · Mycology · Environmental Microbiology
Czapek-Dox Broth
Semi-Synthetic Liquid Medium for Cultivation of Fungi | Cat: AS-1189
A semi-synthetic dehydrated broth formulated for the cultivation and physiological study of fungi capable of utilising nitrate as a nitrogen source. The medium contains sucrose as the carbon source and sodium nitrate as the nitrogen source, making it useful for fungal growth, metabolic and nutrient-utilisation studies.
AS-1189Fungal Culture MediumDehydrated BrothCOA + SDS Available
CatalogueAS-1189
FormatDehydrated Broth
Carbon SourceSucrose
Nitrogen SourceSodium Nitrate
Final pH7.3 ± 0.2
Overview
Czapek-Dox Broth is a semi-synthetic liquid microbiological culture medium used for the cultivation and study of fungi, particularly organisms capable of utilising inorganic nitrate nitrogen.
The formulation provides a controlled nutritional environment with sucrose as the principal carbon source and sodium nitrate as the principal nitrogen source. It is suitable for the cultivation of saprophytic fungi including Aspergillus and Penicillium species, as well as other moulds used in environmental, physiological and metabolic investigations.
Defined nutritional system: The use of defined carbon and nitrogen sources makes Czapek-Dox Broth useful for comparative fungal physiology, carbon-assimilation and nitrogen-utilisation studies.
Applications
| Application | Typical Use |
|---|---|
| Mycology | Cultivation of saprophytic fungi and moulds |
| Aspergillus cultivation | Growth and physiological studies involving Aspergillus species |
| Penicillium cultivation | Growth and metabolic studies involving Penicillium species |
| Environmental microbiology | Study of fungi isolated from soil and environmental samples |
| Fungal physiology | Carbon and nitrogen utilisation, metabolism and comparative growth studies |
Key Features
| Defined carbon source | Sucrose |
| Defined nitrogen source | Sodium nitrate |
| Medium format | Liquid broth |
| Primary application | Fungal cultivation and physiology studies |
| Documentation | TDS, SDS and lot-specific COA available |
Technical Data Sheet Summary
Typical Composition — Per Litre
| Component | Typical Amount | Function |
|---|---|---|
| Sucrose | 30.0 g | Carbon source |
| Sodium Nitrate | 2.0 g | Nitrogen source |
| Dipotassium Phosphate | 1.0 g | Buffer / mineral source |
| Magnesium Sulfate | 0.5 g | Magnesium source |
| Potassium Chloride | 0.5 g | Mineral salt |
| Ferrous Sulfate | 0.01 g | Trace iron source |
| Final pH (25°C) | 7.3 ± 0.2 |
| Dehydrated Appearance | Cream to yellow, homogeneous free-flowing powder |
| Prepared Appearance | Light yellow solution, clear to slightly opalescent |
| Storage | Store tightly closed in a cool, dry place, protected from moisture |
Typical Preparation
| Step 1 | Suspend approximately 34 g of dehydrated medium in 1 L of purified water. |
| Step 2 | Heat gently with agitation until fully dissolved. |
| Step 3 | Dispense into suitable containers. |
| Step 4 | Sterilise by autoclaving at 121°C for 15 minutes. |
| Step 5 | Cool to room temperature before inoculation. |
Preparation note: Follow the concentration, final pH and preparation conditions stated on the current AuSaMicS label, TDS or lot-specific documentation.
Safety Data Sheet Summary
Laboratory product: Handle using standard laboratory hygiene practices and appropriate personal protective equipment.
| Intended Use | Laboratory, research, educational and approved industrial microbiology use only |
| Human / Veterinary Use | Not intended for human or veterinary therapeutic use |
| Handling | Avoid generating or inhaling dust. Avoid contact with eyes and prolonged skin contact. |
| PPE | Laboratory coat, gloves and eye protection recommended during handling and preparation |
| Storage | Keep tightly closed in a cool, dry location protected from moisture |
| Biological Safety | Once inoculated, treat prepared medium as potentially biohazardous and follow institutional biosafety procedures |
| Disposal | Dispose in accordance with local laboratory and biological waste requirements |
Full SDS: Contact support@ausamics.com quoting catalogue number AS-1189.
Certificate of Analysis
Each released batch of Czapek-Dox Broth (AS-1189) can be supplied with a lot-specific Certificate of Analysis documenting applicable finished-product quality checks.
| Test Parameter | Typical Specification / Release Check |
|---|---|
| Dehydrated Appearance | Cream to yellow, homogeneous free-flowing powder |
| Prepared Appearance | Light yellow solution, clear to slightly opalescent |
| Final pH | 7.3 ± 0.2 at 25°C |
| Physical Performance | Acceptable appearance and dissolution after recommended preparation |
| Growth Performance | Expected growth with appropriate fungal reference cultures according to the applicable internal QC method |
| Batch Documentation | Lot number, release data and applicable QC results |
Batch-specific COA: Contact support@ausamics.com quoting catalogue number AS-1189 and the relevant lot number.