CAt No.: AS-1189
Semi-synthetic medium for the cultivation of fungi that utilize inorganic nitrogen, particularly suitable for Aspergillus, Penicillium, and non-sporulating molds.
Store prepared media at temperatures below 8°C and protect it from direct light. Keep dehydrated powder in a dry place, sealed tightly in containers at 2-25°C.
Appearance: Homogeneous, cream to yellow, free-flowing powder.
Color and Clarity: Light yellow solution, clear to slightly opalescent, in tubes.
Czapek-Dox broth
Ingredients Grams/Litre Sucrose 30.0 Sodium Nitrate 3.0 Magnesium Sulfate 0.5 Potassium Chloride 0.5 Potassium Phosphate Dibasic 1.0 Ferrous Sulfate 0.01 Final pH 7.3 +/- 0.2 at 25°C
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Suspend 35.0 g/L of the medium in water.
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Boil the mixture to dissolve the medium completely.
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Sterilize by autoclaving at 121°C for 15 minutes.
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Mix well and dispense as needed.
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